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    <title>topic Re: 4G service with non-4G based tariff in Discussions &amp; Feedback</title>
    <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836338#M58160</link>
    <description>&lt;P&gt;I've never looked at the SIM code, although I can do that before I put it on charge. It has 4GPP2 on the Microsim, meaning the guy must have just done that at the swap-over. Until now, I never ever thought to look. I presume the 3G SIM's have a different code or are unmarked.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
    <pubDate>Sun, 04 Jan 2015 20:46:53 GMT</pubDate>
    <dc:creator>anticpated</dc:creator>
    <dc:date>2015-01-04T20:46:53Z</dc:date>
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      <title>4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836325#M58152</link>
      <description>&lt;P&gt;OK recently, I was in Edinburgh over the holidays to see if there was any sale bargains and what not. I noticed that the signal indicator changed to 4G and the signal was mostly strong. So evidently this means if you have a 4G-capable mobile regardless if you have a 3G tariff, you can still access 4G (LTE) speeds providing you are in the correct area.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;P&gt;What confused me was, a couple of people said to me that you need a 4G SIM and contract to access this type of network. It looks like O2 have updated their networks to work using a hybrid system which will use the fastest transport possible - not restrict you by your SIM type.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;P&gt;I believe this is another grey area where people can tricked into paying more for something they normally can't access. So I don't know this is just an O2 easter egg or the same for all networks however all this means is the 4G tariff merely is just more data allowance not a key to the 4G network.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;P&gt;Anyway I thought it would interesting to mention to anybody with a 4G phone however yet to use the service.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:18:22 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836325#M58152</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T20:18:22Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836326#M58153</link>
      <description>Hi anticpated&lt;BR /&gt;&lt;BR /&gt;That's definitely something I've not come across before.&lt;BR /&gt;&lt;BR /&gt;Yes we are told a person needs&lt;BR /&gt;&lt;BR /&gt;A compatible 4G phone, 4G SIM card and 4G tariff !!</description>
      <pubDate>Sun, 04 Jan 2015 20:21:51 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836326#M58153</guid>
      <dc:creator>Anonymous</dc:creator>
      <dc:date>2015-01-04T20:21:51Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836327#M58154</link>
      <description>I was of the belief that all o2 contracts had been upgraded to 4g now regardless?&lt;BR /&gt;Certainly all mine were, even the 100 min/100mb simo that I have....&lt;BR /&gt;When did you last do a sim swap? If your sim serial number starts with 4ggp then it is a 4g sim card already so would explain you receiving a 4g signal.</description>
      <pubDate>Sun, 04 Jan 2015 20:29:33 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836327#M58154</guid>
      <dc:creator>MI5</dc:creator>
      <dc:date>2015-01-04T20:29:33Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836332#M58155</link>
      <description>&lt;P&gt;So...let me check...I have a 4G phone with an old 3G sim in ....Are you saying that if I travel to a 4G area I will get 4G? If so, I never noticed that when I went to Coventry and Birmingham before Xmas...?&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:38:34 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836332#M58155</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T20:38:34Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836333#M58156</link>
      <description>&lt;P&gt;No. You will not get 4G with a 3G sim.&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:40:42 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836333#M58156</guid>
      <dc:creator>Bambino</dc:creator>
      <dc:date>2015-01-04T20:40:42Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836334#M58157</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/14600"&gt;@Bambino&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;No. You will not get 4G with a 3G sim.&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;Correct - I am guessing &lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt;&amp;nbsp;has a 4g sim already.&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:44:33 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836334#M58157</guid>
      <dc:creator>MI5</dc:creator>
      <dc:date>2015-01-04T20:44:33Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836336#M58158</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/14600"&gt;@Bambino&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;No. You will not get 4G with a 3G sim.&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;I didnt think so...but &lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt;&amp;nbsp; didn't specify what sim was in his phone?&lt;/P&gt;&lt;P&gt;I understand what he is saying about getting 4G whilst I presume he must be on a 3G tariff....but I just wanted all the info&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:45:48 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836336#M58158</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T20:45:48Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836337#M58159</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/24643"&gt;@MI5&lt;/a&gt; wrote:&lt;BR /&gt;&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/14600"&gt;@Bambino&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;No. You will not get 4G with a 3G sim.&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;Correct - I am guessing &lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt;&amp;nbsp;has a 4g sim already.&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;But on a 3G tariff then?&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:46:33 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836337#M58159</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T20:46:33Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836338#M58160</link>
      <description>&lt;P&gt;I've never looked at the SIM code, although I can do that before I put it on charge. It has 4GPP2 on the Microsim, meaning the guy must have just done that at the swap-over. Until now, I never ever thought to look. I presume the 3G SIM's have a different code or are unmarked.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:46:53 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836338#M58160</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T20:46:53Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836339#M58161</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;I've never looked at the SIM code, although I can do that before I put it on charge. It has 4GPP2 on the Microsim, meaning the guy must have just done that at the swap-over. Until now, I never ever thought to look. I presume the 3G SIM's have a different code or are unmarked.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;So you have a 4G sim but a 3G tariff...??&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 20:48:44 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836339#M58161</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T20:48:44Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836341#M58162</link>
      <description>All o2 tariffs are now 4g</description>
      <pubDate>Sun, 04 Jan 2015 20:59:48 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836341#M58162</guid>
      <dc:creator>Anonymous</dc:creator>
      <dc:date>2015-01-04T20:59:48Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836342#M58163</link>
      <description>He has a 4g sim and a 4g tariff - All O2 tariffs have been upgraded automatically so will receive 4g when in a 4g area.</description>
      <pubDate>Sun, 04 Jan 2015 21:00:28 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836342#M58163</guid>
      <dc:creator>MI5</dc:creator>
      <dc:date>2015-01-04T21:00:28Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836343#M58164</link>
      <description>&lt;P&gt;Sorry for any confusion there although as you can I was imagine, before I properly investigated was a bit surprised by the situation. It's not like O2 to do something which is actually quite surprisingly good and at the same time misleading. &lt;span class="lia-unicode-emoji" title=":winking_face:"&gt;😉&lt;/span&gt;&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;P&gt;Anyway for around £6 a month if I could get 250MB more data allowance. Being as my local area isn't 4G savvy it would be a waste of time however at least I know when it is I should get it without changing SIM.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;P&gt;Thanks for the responses - welcome to the recesses of my mind!&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:00:37 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836343#M58164</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T21:00:37Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836344#M58165</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;I presume the 3G SIM's have a different code or are unmarked.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;Surprisingly they start 3ggp &lt;img class="lia-deferred-image lia-image-emoji" src="https://community.o2.co.uk/html/@0298759A4703928FCB017542757CF0DA/images/smilies/013.png" alt="wink" title="wink" /&gt;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:01:17 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836344#M58165</guid>
      <dc:creator>MI5</dc:creator>
      <dc:date>2015-01-04T21:01:17Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836347#M58166</link>
      <description>&lt;P&gt;At least now, I will armed with this information when the vultures swoop the next time I'm in a mobile shop.&amp;nbsp;:mantongue:&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:02:37 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836347#M58166</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T21:02:37Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836349#M58167</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/24643"&gt;@MI5&lt;/a&gt; wrote:&lt;BR /&gt;He has a 4g sim and a 4g tariff - All O2 tariffs have been upgraded automatically so will receive 4g when in a 4g area.&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;Well, even I would expect to get that....So not an innovative thing for me to try... I thought for one minute I needn't bother swapping sims or tariffs &lt;img id="happy-smiley58" class="emoticon emoticon-happy-smiley58" src="https://community.o2.co.uk/html/@401597C226859A9A1B62A3B67621FD5E/images/smilies/happy-smiley58.gif" alt="LOL" title="LOL" /&gt;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:04:41 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836349#M58167</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T21:04:41Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836351#M58168</link>
      <description>&lt;P&gt;3GGP well I never. I thought that was a chemical compound.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:05:00 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836351#M58168</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T21:05:00Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836352#M58169</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;3GGP well I never. I thought that was a chemical compound.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;&lt;img id="happy-smiley58" class="emoticon emoticon-happy-smiley58" src="https://community.o2.co.uk/html/@401597C226859A9A1B62A3B67621FD5E/images/smilies/happy-smiley58.gif" alt="LOL" title="LOL" /&gt;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:05:39 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836352#M58169</guid>
      <dc:creator>Cleoriff</dc:creator>
      <dc:date>2015-01-04T21:05:39Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836355#M58170</link>
      <description>&lt;P&gt;Call me stupid if you like however this was not explained to me at the time when the SIM swap took place. Then I got the O2 message about the network upgrade. It just nice to know that I'm on the same page right now.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:08:13 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836355#M58170</guid>
      <dc:creator>anticpated</dc:creator>
      <dc:date>2015-01-04T21:08:13Z</dc:date>
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      <title>Re: 4G service with non-4G based tariff</title>
      <link>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836357#M58171</link>
      <description>&lt;BLOCKQUOTE&gt;&lt;HR /&gt;&lt;a href="https://community.o2.co.uk/t5/user/viewprofilepage/user-id/25387"&gt;@anticpated&lt;/a&gt; wrote:&lt;BR /&gt;&lt;P&gt;3GGP well I never. I thought that was a chemical compound.&lt;/P&gt;&lt;P&gt;&amp;nbsp;&lt;/P&gt;&lt;HR /&gt;&lt;/BLOCKQUOTE&gt;&lt;P&gt;Oh, are you talking about,&amp;nbsp;&lt;SPAN&gt;&lt;A&gt;TyrA&lt;/A&gt; &lt;A&gt;proteins&lt;/A&gt; &lt;A&gt;belong&lt;/A&gt; to a &lt;A&gt;family&lt;/A&gt; of &lt;A&gt;dehydrogenases&lt;/A&gt; that are &lt;A&gt;dedicated&lt;/A&gt; to &lt;A&gt;l-tyrosine&lt;/A&gt; &lt;A&gt;biosynthesis.&lt;/A&gt; The &lt;A&gt;three&lt;/A&gt; &lt;A&gt;TyrA&lt;/A&gt; &lt;A&gt;subclasses&lt;/A&gt; are&lt;A&gt;distinguished&lt;/A&gt; by their &lt;A&gt;substrate&lt;/A&gt; &lt;A&gt;specificities,&lt;/A&gt; &lt;A&gt;namely&lt;/A&gt; the &lt;A&gt;prephenate&lt;/A&gt; &lt;A&gt;dehydrogenases,&lt;/A&gt; the &lt;A&gt;arogenate&lt;/A&gt; &lt;A&gt;dehydrogenases,&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; the&lt;A&gt;cyclohexadienyl&lt;/A&gt; &lt;A&gt;dehydrogenases,&lt;/A&gt; &lt;A&gt;which&lt;/A&gt; &lt;A&gt;utilize&lt;/A&gt; &lt;A&gt;prephenate,&lt;/A&gt; &lt;A&gt;l-arogenate,&lt;/A&gt; or &lt;A&gt;both&lt;/A&gt; &lt;A&gt;substrates,&lt;/A&gt; &lt;A&gt;respectively.&lt;/A&gt; The &lt;A&gt;molecular&lt;/A&gt;&lt;A&gt;mechanism&lt;/A&gt; &lt;A&gt;responsible&lt;/A&gt; for &lt;A&gt;TyrA&lt;/A&gt; &lt;A&gt;substrate&lt;/A&gt; &lt;A&gt;selectivity&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;regulation&lt;/A&gt; is &lt;A&gt;unknown.&lt;/A&gt; To &lt;A&gt;further&lt;/A&gt; &lt;A&gt;our&lt;/A&gt; &lt;A&gt;understanding&lt;/A&gt; of &lt;A&gt;TyrA-catalyzed&lt;/A&gt;&lt;A&gt;reactions,&lt;/A&gt; &lt;A&gt;we&lt;/A&gt; &lt;A&gt;have&lt;/A&gt; &lt;A&gt;determined&lt;/A&gt; the &lt;A&gt;crystal&lt;/A&gt; &lt;A&gt;structures&lt;/A&gt; of &lt;A&gt;Aquifex&lt;/A&gt; &lt;A&gt;aeolicus&lt;/A&gt; &lt;A&gt;prephenate&lt;/A&gt; &lt;A&gt;dehydrogenase&lt;/A&gt; &lt;A&gt;bound&lt;/A&gt; with &lt;A&gt;NAD(+)&lt;/A&gt; &lt;A&gt;plus&lt;/A&gt;&lt;A&gt;either&lt;/A&gt; &lt;A&gt;4-hydroxyphenylpyuvate,&lt;/A&gt; &lt;A&gt;4-hydroxyphenylpropionate,&lt;/A&gt; or &lt;A&gt;l-tyrosine&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;have&lt;/A&gt; &lt;A&gt;used&lt;/A&gt; these &lt;A&gt;structures&lt;/A&gt; as &lt;A&gt;guides&lt;/A&gt; to &lt;A&gt;target&lt;/A&gt; &lt;A&gt;active&lt;/A&gt;&lt;A&gt;site&lt;/A&gt; &lt;A&gt;residues&lt;/A&gt; for &lt;A&gt;site-directed&lt;/A&gt; &lt;A&gt;mutagenesis.&lt;/A&gt; &lt;A&gt;From&lt;/A&gt; a &lt;A&gt;combination&lt;/A&gt; of &lt;A&gt;mutational&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;structural&lt;/A&gt; &lt;A&gt;analyses,&lt;/A&gt; &lt;A&gt;we&lt;/A&gt; &lt;A&gt;have&lt;/A&gt; &lt;A&gt;demonstrated&lt;/A&gt; that&lt;A&gt;His-147&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;Arg-250&lt;/A&gt; are &lt;A&gt;key&lt;/A&gt; &lt;A&gt;catalytic&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;binding&lt;/A&gt; &lt;A&gt;groups,&lt;/A&gt; &lt;A&gt;respectively,&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;Ser-126&lt;/A&gt; &lt;A&gt;participates&lt;/A&gt; in &lt;A&gt;both&lt;/A&gt; &lt;A&gt;catalysis&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; &lt;A&gt;substrate&lt;/A&gt;&lt;A&gt;binding&lt;/A&gt; &lt;A&gt;through&lt;/A&gt; the &lt;A&gt;ligand&lt;/A&gt; &lt;A&gt;4-hydroxyl&lt;/A&gt; &lt;A&gt;group.&lt;/A&gt; The &lt;A&gt;crystal&lt;/A&gt; &lt;A&gt;structure&lt;/A&gt; &lt;A&gt;revealed&lt;/A&gt; that &lt;A&gt;tyrosine,&lt;/A&gt; a &lt;A&gt;known&lt;/A&gt; &lt;A&gt;inhibitor,&lt;/A&gt; &lt;A&gt;binds&lt;/A&gt; &lt;A&gt;directly&lt;/A&gt; to the&lt;A&gt;active&lt;/A&gt; &lt;A&gt;site&lt;/A&gt; of the &lt;A&gt;enzyme&lt;/A&gt; &lt;A&gt;and&lt;/A&gt; not to &lt;A&gt;an&lt;/A&gt; &lt;A&gt;allosteric&lt;/A&gt; &lt;A&gt;site.&lt;/A&gt; The &lt;A&gt;most&lt;/A&gt; &lt;A&gt;interesting&lt;/A&gt; &lt;A&gt;finding&lt;/A&gt; &lt;A&gt;though,&lt;/A&gt; is that &lt;A&gt;mutating&lt;/A&gt; &lt;A&gt;His-217&lt;/A&gt; &lt;A&gt;relieved&lt;/A&gt; the&lt;A&gt;inhibitory&lt;/A&gt; &lt;A&gt;effect&lt;/A&gt; of &lt;A&gt;tyrosine&lt;/A&gt; on &lt;A&gt;A.&lt;/A&gt; &lt;A&gt;aeolicus&lt;/A&gt; &lt;A&gt;prephenate&lt;/A&gt; &lt;A&gt;dehydrogenase.&lt;/A&gt; The &lt;A&gt;identification&lt;/A&gt; of a &lt;A&gt;tyrosine-insensitive&lt;/A&gt; &lt;A&gt;mutant&lt;/A&gt; &lt;A&gt;provides&lt;/A&gt; a&lt;A&gt;novel&lt;/A&gt; &lt;A&gt;avenue&lt;/A&gt; for &lt;A&gt;designing&lt;/A&gt; &lt;A&gt;an&lt;/A&gt; &lt;A&gt;unregulated&lt;/A&gt; &lt;A&gt;enzyme&lt;/A&gt; for &lt;A&gt;application&lt;/A&gt; in &lt;A&gt;metabolic&lt;/A&gt; &lt;A&gt;engineering ?&lt;/A&gt;&lt;/SPAN&gt;&lt;/P&gt;</description>
      <pubDate>Sun, 04 Jan 2015 21:09:18 GMT</pubDate>
      <guid>https://community.o2.co.uk/t5/Discussions-Feedback/4G-service-with-non-4G-based-tariff/m-p/836357#M58171</guid>
      <dc:creator>MI5</dc:creator>
      <dc:date>2015-01-04T21:09:18Z</dc:date>
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